If there's some chance that I already have HSV1 and can stop worrying about catching it, I think the psychological relief would be worth the money. But that's proved harder than I thought - a year out, I'm still having anxiety which is starting to ruin our sex life. Initially I decided not to bother spending money on the Western Blot since HSV1 is not a big deal and figured I'd just risk it. I developed strange symptoms but, my blood tests have consistently been negative. To recap, my boyfriend was diagnosed (culture and blood) with G-HSV1. Sexually Transmitted Diseases, 44-12:763-767.Dear Terri, Awhile back, you suggested that I get a Western Blot test for HSV-1 ( /posts/Herpes/Boyfriend-has-G-HSV1-please-advise/show/1802921#post_8304417). Performance of Commercial Enzyme-linked Immunoassays for Diagnosis of Herpes Simplex Virus-1 and Herpes Simplex Virus-2 Infection in a Clinical Setting. It was reported that nearly 50% of commercial tests indicating an HSV-2 infection were inaccurate 7 Agyemang, et al, 2017. For this reason, a report from the American Sexual Health Association in 2017 states that improved Herpes diagnostic tests are needed. Additional economically feasible, reliable assays which are not as technically challenging, should be welcomed in the market place.ĬONCLUSION: Commercial tests for the detection of HSV are often unreliable, especially for those with a “low positive” score. Poor specificity of this test in HIV-1 seropositive patients shows a need for additional commercially available for HSV-2 detection in these populations. These investigators found that the high false positivity rate of the HerpeSelect assay was a challenge in African sera. Performance of commercial Herpes Simplex Virus Type-2 antibody tests using serum samples from Sub-Sahara Africa: a systematic review and meta-analysis. Both of these assays have optimal accuracy in industrialized countries, while their performance on sera from developing countries has varied 6 Biraro, et al., 2011. While the University of Washington HSC Western blot (UW-WB) is expensive and technically difficult for screening and clinical trials, other commercial assays such as the Kalon (which is not yet FDA approved) and the FDA-cleared Focus HerpeSelct-2 IgG ELISA offer reliable assays for use in secondary research. found that the Kalon assay was reliable, stand-alone, on-site assay for detecting HSV-2 IgG antibodies. al., 5 Patel, et al., Precision of the Kalon Herpes Simplex Virus Type 2 IgG ELISA: an international inter-laboratory assessment. The tests which performed the best were the Gull Premier EIA (sensitivity, 86.7% specificity 97.6%), and the Kalon Biological (sensitivity, 92.3% specificity, 97.7%) tests. Test sensitivities from 86% to 100%, while the specificity ranged from 47-99%. The discordant assays and the three best assays were compared to the Western blot assay from the University of Washington. Test samples were first compared to monoclonal antibody (MAb) enzyme immunoassays (EIA) from the Central Public Health Lab, London, UK. Performance of Commercially Available Enzyme Immunoassays for Detection of Antibodies against Herpes Simplex Virus Type 2 in African Populations. One review compared thirteen available commercial immunoassays for HSV-2 4 van Dyck, et al., 2004. DISCUSSION: Several reports have analyzed available commercial assays in the past. Sexually Transmitted Diseases Treatment Guidelines. False-positive results can bring emotional stress to the patient, and for this reason, a second test is often requested for a confirmatory result. The inaccuracy of Certain Commercial Enzyme Immunoassays in Diagnosing Genital Infections with Herpes Simplex Virus Types 1 and 2.
While this FDA approved assay is considered to be very accurate, a second confirmatory assay is often preferred as most patients are unaware of their genital Herpes status 2 Ashley-Morrow, Rhoda, and David Friedrich, 2003. Of these, the University of Washington Western blot assay (UW-WB) is considered the “gold standard” for assays to detect Herpes virus antibodies in patient sera 1 University of Washington Western blot. INTRODUCTION: There are several commercial immunoassays for HSV on the market (Western blot, Dot blot or standard ELISA assays). By David Kilpatrick, PhD and Abbas Vafai, PhD
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